Protein Data Bank. Skip to main content. Select a section on the left to see content. Pfam protein domain database More It was soon after established that the emission spectra of GFP was right around nm, or the ultraviol A dinoflagellate luciferase is capable of emitting light due to its interaction with its substrate luciferin and the luciferin-binding protein LBP in the scintillon organelle found in dinoflagellates.
luc. Organism. Photinus pyralis (Common eastern firefly) (Lampyris pyralis). Status -Protein predictedi.
Video: Firefly luciferase protein sequences Applying luciferin substrate to glowing plants
This indicates the type of. Sequence databases. Firefly luciferase is a 62 kDa protein that catalyzes the production of light. In the presence of Find similar proteins by: Sequence | Structure. The N-terminal amino acid sequences of the firefly luciferase are important for firefly luciferase in the yeast Saccharomyces cerevisiae as a fusion protein to.
The gene contains six introns, all less than 60 bases in length.
luc Firefly luciferase Photinus pyralis (Common eastern firefly) luc gene & protein
Gene3D i 4. The firefly luciferase activity of a series of deletion and site-directed mutants in the amino-terminal region was investigated in vitro and in vivo. Oxidoreductases : monooxygenases EC 1. These are stable identifiers and should be used to cite UniProtKB entries.
Database of comparative protein structure models More Effect of solvents on the catalytic activity of firefly luciferase.
Organization and expression of eucaryotic split genes coding for proteins.
Recombinant Firefly Luciferase Protein (NBP) Novus Biologicals
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined expression plasmid and expressed a fusion protein in E. coli.
Video: Firefly luciferase protein sequences Luciferase Assay
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, One well-studied luciferase is that of the Photinini firefly Photinus pyralis, which has an optimum pH of. by increasing the luminescence intensity by changing certain amino acid residues in the sequence of the protein.
Primary structure of a chloramphenicol acetyltransferase specified by R plasmids.
Mutation of a Proteasesensitive Region in Firefly Luciferase Alters Light Emission Properties
Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. ChEMBL i. EC number Enzyme superfamily Enzyme family List of enzymes. View Pathways.
RCSB PDB 1LCI FIREFLY LUCIFERASE
Firefly luciferase protein sequences
|The opportunities for using luciferase continue to expand.
On the other hand, mutant Asn6Lys retained the wild-type activity level. Quick Order. Measuring gene expression with light. Termination-reinitiation occurs in the translation of mammalian cell mRNAs.