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Journal of Quantitative Spectroscopy and Radiative Transfer Opt Lett ; 40 : — Note that the chloroplasts which correspond to the fluorescence part of the cell represent only a fraction of the entire volume. However, in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several directions. In conclusion, the R-TPM approach is a single-cell analysis tool that allows the study of hundreds of cells and supplies as output their complete morphologic classification in 3D via the self-rotation of cells in microfluidic channels. Various classes of tomographic concepts exist, such as X-ray computed tomography, optical coherence tomography 23 and tomographic phase microscopy TPM 45678910111213 Sign up to take part. Biomed Opt Express ; 6 : — Results and discussion The working conditions and the adopted optical system are depicted in Figure 1a.

  • Tomographic flow cytometry by digital holography Light Science & Applications

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    It has been demonstrated that in the presence of these elements, chloroplasts show signs of structural rupture or even disintegration. Journal of Biomedical Optics Developing optofluidic technology through the fusion of microfluidics and optics.

    An accurate characterization of both types of cells is reported, despite their very different nature and material content, thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.

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    Science ; : — Not all typefaces shown. Red blood cell morphology.

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    Figaro Furthermore, we demonstrate that a priori knowledge of the rotation angle in 3D is no longer necessary because it can be accurately retrieved from the intelligent processing of the QPMs.

    More details regarding the optical set-up are given in Materials and methods.

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    Tomographic flow cytometry by digital holography Light Science & Applications

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    In fact, RBCs and DAs have a crucial role in the health of the human body and our planet, respectively.

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    Figure 2b shows one of the RBCs analyzed for the healthy sample in hypertonic solution, where the shape modification, as induced by the medium, provides the typical burr shape.

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    In conclusion, the R-TPM approach is a single-cell analysis tool that allows the study of hundreds of cells and supplies as output their complete morphologic classification in 3D via the self-rotation of cells in microfluidic channels.

    Under our experimental conditions, the deformations of RBCs are negligible compared to the rotations they undergo, and the approximation of a rigid body is achieved 3645 see Materials and methods for details.

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    Highly concentrated cultures were diluted with filtered 0.

    Nimrod Arial Footlight Calisto Cantoria. The tomographic reconstruction allows us to exactly identify their location and dimensions. In our case, different from Cotte et al. Hum Mutat ; 35 : —